| 1 | 17 | June-Reg | C.J. Henry | ss | 19 | 6-3 | 205 | R | R | A+ | | Putnam City (Oklahoma City,OK) | $1,575,000 |
| 2 | 63 | June-Reg | J.B. Cox | P | 21 | 6-3 | 205 | L | R | AAA | | University of Texas | $550,000 |
| 3 | 109 | June-Reg | Brett Gardner | OF | 21 | 5-10 | 180 | L | L | MLB | 2008-2009 | College of Charleston | $210,000 |
| 4 | 139 | June-Reg | Lance Pendleton | P | 21 | 6-3 | 195 | L | R | AA | | Rice University | $215,000 |
| 5 | 169 | June-Reg | Zach Kroenke | P | 21 | 6-3 | 210 | R | L | AAA | | University of Nebraska | $155,000 |
| 6 | 199** | June-Reg | Doug Fister | P | 21 | 6-8 | 185 | L | R | MLB | 2009-2009 | Fresno State University | $0 |
| 7 | 229 | June-Reg | Garrett Patterson | P | 23 | 6-0 | 222 | L | L | A+ | | University of Oklahoma | $90,000 |
| 8 | 259 | June-Reg | Austin Jackson | OF | 18 | 6-1 | 185 | R | R | AAA | | Ryan (Denton,TX) | $800,000 |
| 9 | 289 | June-Reg | James Cooper | of | 21 | 5-10 | 190 | L | R | AA | | Loyola Marymount University | $60,000 |
| 10 | 319 | June-Reg | Kyle Anson | 3B | 22 | 6-0 | 200 | B | R | AA | | Texas State University | $10,000 |
| 11 | 349 | June-Reg | Alan Horne | P | 22 | 6-4 | 195 | R | R | AAA | | University of Florida | |
| 12 | 379 | June-Reg | Joe Muich | c | 22 | 6-1 | 205 | R | R | AA | | Wichita State University | |
| 13 | 409 | June-Reg | Karl Amonite | 1b | 23 | 6-5 | 235 | L | R | College | | Auburn University | |
| 14 | 439 | June-Reg | Joel Perez | OF | 23 | 5-10 | 190 | R | R | Rookie | | | |
| 15 | 469 | June-Reg | Josh Schmidt | P | 22 | 6-4 | 175 | R | R | AA | | University of the Pacific | |
| 16 | 499 | June-Reg | Chris Malec | 2b | 22 | 5-11 | 195 | B | R | AAA | | University of California-Santa Barbara | |
| 17 | 529 | June-Reg | Keaton Everitt | P | 22 | 6-7 | 220 | R | R | A | | University of Washington | |
| 18 | 559 | June-Reg | Joe Burke | c | 21 | 6-0 | 195 | L | R | A+ | | St. John's University | |
| 19 | 589 | June-Reg | Jim Conroy | P | 22 | 6-3 | 190 | R | R | A | | University of Illinois | |
| 20 | 619 | June-Reg | Bryan Rueger | P | 22 | 6-3 | 185 | L | L | A+ | | Southern Illinois University | |
| 21 | 649 | June-Reg | Hanseld Diaz | C | 20 | 6-1 | 200 | R | R | Ind | | Miami-Dade College | |
| 22 | 679** | June-Reg | Michael Lee | P | 18 | 6-7 | 220 | R | R | AA | | Skyline (Sammamish,WA) | |
| 23 | 709 | June-Reg | Matt Wallach | c | 19 | 6-2 | 210 | L | R | A | | Cypress College | |
| 24 | 739 | June-Reg | Justin Phillabaum | 1B | 19 | 6-3 | 210 | R | R | A+ | | Indian River State College | |
| 25 | 769 | June-Reg | Brad Canada | c | 22 | 6-1 | 210 | R | R | A- | | Bradley University | |
| 26 | 799 | June-Reg | Patrick Caldwell | P | | | | | | - | | Yavapai College | |
| 27 | 829** | June-Reg | Joshua Morgan | P | 20 | 6-2 | 200 | R | R | - | | University of Missouri-St. Louis | |
| 28 | 859 | June-Reg | Derek Duclos | P | 22 | 6-4 | 220 | R | R | Ind | | University of Richmond | |
| 29 | 889** | June-Reg | Justin Turner | 2B | 20 | 5-11 | 180 | R | R | MLB | 2009-2009 | Cal State Fullerton | |
| 30 | 919 | June-Reg | Cameron Johnson | of | 18 | 6-2 | 210 | R | R | College | | College of Southern Nevada | |
| 31 | 949 | June-Reg | Clint Preisendorfer | P | 18 | 6-5 | 235 | L | L | Rookie | | Palomar College | |
| 32 | 979 | June-Reg | Jared Souza | P | 20 | 6-3 | 198 | L | L | - | | Navarro College | |
| 33 | 1009 | June-Reg | Michael Miotkowski | P | 23 | 6-5 | 245 | R | R | - | | Oklahoma City University | |
| 34 | 1039 | June-Reg | Felipe Garcia | 1B | 22 | 6-3 | 225 | R | R | A+ | | Cal State Fullerton | |
| 35 | 1069 | June-Reg | Andy Carter | P | 22 | 6-4 | 215 | R | R | Ind | | Davidson College | |
| 36 | 1099** | June-Reg | Brett Summers | P | 18 | 6-6 | 185 | R | R | - | | Lake Central (St. John,IN) | |
| 37 | 1129 | June-Reg | Jesse Bahr | P | 22 | 6-2 | 200 | L | L | A- | | Missouri Baptist University | |
| 38 | 1159 | June-Reg | Clay Young | P | 19 | 6-5 | 220 | L | L | - | | Glendale Community College | |
| 39 | 1188 | June-Reg | Miguel Ruiz | P | 18 | 6-2 | 188 | R | R | AAA | | South Hills (Fort Worth,TX) | |
| 40 | 1217 | June-Reg | Michael Roskopf | 1B | 19 | 6-7 | 220 | L | R | College | | College of Southern Nevada | |
| 41 | 1246 | June-Reg | Chris Valencia | of | 18 | 6-1 | 195 | L | R | - | | Westchester (Los Angeles,CA) | |
| 42 | 1275 | June-Reg | Josh Styes | P | 18 | 6-3 | 190 | R | R | - | | Clay Chalkville (Pinson,AL) | |
| 43 | 1304 | June-Reg | Brady Salter | P | 22 | 6-5 | 220 | R | R | Ind | | Aurora University | |
| 44 | 1333 | June-Reg | Justin Keadle | P | 22 | 6-2 | 211 | R | R | A- | | Wake Forest University | |
| 45 | 1362** | June-Reg | Brett Pill | 1B | 20 | 6-4 | 215 | R | R | AA | | Cal State Fullerton | |
| 46 | 1391 | June-Reg | Eric Wordekemper | P | 21 | 6-1 | 200 | R | R | AAA | | Creighton University | |
| 47 | 1419 | June-Reg | Eric Hanlon | of | 18 | 6-3 | 185 | L | R | College | | Martin (Arlington,TX) | |
| 48 | 1447 | June-Reg | Andrew Rice | of | 20 | 6-1 | 195 | L | L | - | | Itawamba Community College | |
| 49 | 1474 | June-Reg | Diallo Fon | of | 18 | 6-0 | 185 | L | L | College | | Las Lomas (Walnut Creek,CA) | |
| 50 | 1501 | June-Reg | Blake Heym | C | 20 | 6-2 | 195 | L | R | - | | Grayson County College | |