| 1 | 4 | June-Reg | Jeff Niemann | P | 21 | 6-9 | 260 | R | R | MLB | 2008-2009 | Rice University | $3,200,000 |
| 2 | 45 | June-Reg | Reid Brignac | SS | 18 | 6-3 | 170 | L | R | MLB | 2008-2009 | St. Amant (St. Amant,LA) | $795,000 |
| 3 | 75 | June-Reg | Wade Davis | P | 18 | 6-5 | 220 | R | R | MLB | 2009-2009 | Lake Wales (Lake Wales,FL) | $475,000 |
| 4 | 105 | June-Reg | Matt Spring | C | 19 | 6-2 | 215 | R | R | AA | | Dixie State College of Utah | $350,000 |
| 5 | 135 | June-Reg | Jake McGee | P | 17 | 6-3 | 190 | L | L | AA | | Reed (Sparks,NV) | $215,000 |
| 6 | 165 | June-Reg | Ryan Royster | OF | 17 | 6-2 | 205 | R | R | A+ | | Churchill (Eugene,OR) | $160,000 |
| 7 | 195 | June-Reg | Fernando Perez | OF | 21 | 6-1 | 195 | R | R | MLB | 2008-2009 | Columbia University | $160,000 |
| 8 | 225 | June-Reg | Rhyne Hughes | 1B | 20 | 6-2 | 175 | L | L | AAA | | Pearl River Community College | $275,000 |
| 9 | 255 | June-Reg | Joseph Muro | P | 20 | 6-0 | 161 | R | R | A- | | Mount San Antonio College | $60,000 |
| 10 | 285 | June-Reg | Matt Walker | P | 17 | 6-3 | 193 | R | R | A+ | | Central (Baton Rouge,LA) | $600,000 |
| 11 | 315 | June-Reg | Josh Asanovich | 2B | 21 | 6-0 | 190 | R | R | AA | | Arizona State University | |
| 12 | 345 | June-Reg | Chris Cunningham | OF | 21 | 6-0 | 200 | R | R | A+ | | South Suburban College | |
| 13 | 375 | June-Reg | Andy Sonnanstine | P | 21 | 6-3 | 185 | L | R | MLB | 2007-2009 | Kent State University | |
| 14 | 405 | June-Reg | Woods Fines | P | 18 | 6-5 | 190 | R | R | A | | Northwood (Pittsboro,NC) | |
| 15 | 435 | June-Reg | Kenneth Brock | P | 21 | 6-1 | 220 | L | L | Rookie | | Lane College | |
| 16 | 465** | June-Reg | Cale Iorg | SS | 18 | 6-2 | 175 | R | R | AA | | Karns (Knoxville,TN) | |
| 17 | 495 | June-Reg | Marcus Barriger | P | 21 | 6-1 | 215 | R | R | A- | | Armstrong Atlantic State University | |
| 18 | 525 | June-Reg | James Scholzen | SS | | | | | | Rookie | | Hurricane (Hurricane,UT) | |
| 19 | 555 | June-Reg | Chris Nowak | 3B | 21 | 6-5 | 225 | R | R | AAA | | University of South Carolina-Upstate | |
| 20 | 585** | June-Reg | Matt Duryea | P | | | | | | - | | Marshfield (Coos Bay,OR) | |
| 21 | 615 | June-Reg | Patrick Breen | OF | 22 | 6-3 | 210 | L | L | AA | | University of Houston | |
| 22 | 645 | June-Reg | Ryan Bitter | P | 22 | 6-1 | 195 | R | R | A | | Siena College | |
| 23 | 675 | June-Reg | Logan Wiens | 1B | 18 | 6-6 | 210 | R | R | A | | Merced (Merced,CA) | |
| 24 | 705 | June-Reg | Francisco Leandro | OF | 23 | 5-10 | 180 | L | L | AA | | University of Central Missouri | |
| 25 | 735** | June-Reg | Deunte Heath | P | 18 | 6-4 | 205 | R | R | AAA | | Lake City Community College | |
| 26 | 765 | June-Reg | Alex Crooks | 1B | 20 | 6-2 | 235 | L | L | Rookie | | East Los Angeles College | |
| 27 | 795** | June-Reg | Matt Goyen | P | 21 | 6-5 | 220 | R | L | A+ | | Georgia College & State University | |
| 28 | 825 | June-Reg | Nick Wagner | P | 21 | 6-2 | 220 | R | R | A | | Georgia Tech | |
| 29 | 855** | June-Reg | Daniel McCutchen | P | 21 | 6-1 | 195 | R | R | MLB | 2009-2009 | University of Oklahoma | |
| 30 | 885 | June-Reg | Aaron Walker | P | 22 | 6-3 | 198 | L | L | A+ | | Georgia Tech | |
| 31 | 915 | June-Reg | John Rodriguez | P | | | | | | - | | Pearl River Community College | |
| 32 | 945 | June-Reg | Matt Rainey | P | 20 | 6-0 | 200 | R | R | A- | | Odessa College | |
| 33 | 975 | June-Reg | Patrick Cottrell | SS | 22 | 5-11 | 160 | R | R | AA | | University of West Florida | |
| 34 | 1005 | June-Reg | Grant Theophilus | P | 19 | 6-4 | 220 | L | L | College | | Cypress College | |
| 35 | 1035 | June-Reg | Billy Evers | P | 21 | 6-2 | 195 | R | R | A | | Eckerd College | |
| 36 | 1065 | June-Reg | Ryan Davis | 3B | 18 | 6-1 | 200 | L | R | College | | Chaparral (Scottsdale,AZ) | |
| 37 | 1095 | June-Reg | Brian McCormick | C | | | | | | - | | Arundel (Gambrills,MD) | |
| 38 | 1125 | June-Reg | Ryan Conan | SS | 18 | | | | | College | | Archbishop Mitty (San Jose,CA) | |
| 39 | 1155 | June-Reg | Drew Bigda | P | 21 | 6-0 | 220 | R | L | A | | College of the Holy Cross | |
| 40 | 1185 | June-Reg | Jason Dean | OF | 19 | 5-11 | 171 | R | R | - | | Sacramento City College | |
| 41 | 1215 | June-Reg | J.T. Hall | OF | 20 | 6-3 | 210 | L | R | AAA | | Southwest Mississippi Community College | |
| 42 | 1244 | June-Reg | Matt Ware | P | | | | | | - | | Quartz Hill (Quartz Hill,CA) | |
| 43 | 1273 | June-Reg | John Price | P | 22 | 6-2 | 220 | R | L | A- | | University of Mobile | |
| 44 | 1302 | June-Reg | Matt Fields | 1B | 18 | 6-4 | 255 | R | R | AA | | Rainier Beach (Seattle,WA) | |
| 45 | 1331 | June-Reg | John Bouman | SS | 21 | 6-4 | 190 | R | R | A+ | | Tusculum College | |
| 46 | 1360 | June-Reg | Chris Kelly | P | 21 | 6-3 | 200 | R | R | AA | | Jacksonville University | |
| 47 | 1389 | June-Reg | Ben Lanier | SS | | | | | | - | | Kinston (Kinston,NC) | |
| 48 | 1418 | June-Reg | Andrew Gray | 1B | | | | | | - | | Holy Spirit (Tuscaloosa,AL) | |
| 49 | 1446 | June-Reg | Patrick Mahoney | C | | | | | | A- | | Lincoln (Des Moines,IA) | |
| 50 | 1474 | June-Reg | Jerrylee Scott | SS | | | | | | - | | Livonia (Livonia,LA) | |