| # | Player Name | Pos | Team | Age | Born | Ht | Wt | B-T | High Level | MLB Years | Drafted From | # |
| 578. | Mark Schleicher | 2B | TB | 21 | 1/1/1982 | 6-1 | 180 | R-R | AA | | Pfeiffer University | 15 |
| 579. | Nick Slack | P | MIL | 20 | 1/5/1983 | 6-1 | 215 | R-R | A+ | | Lake City Community College | 23 |
| 580. | Nick McIntyre | 2B | DET | 22 | 3/11/1981 | 5-10 | 195 | B-R | AA | | Purdue University | 40 |
| 581. | Leo Rosales | P | SD | 22 | 5/28/1981 | 6-1 | 190 | R-R | AAA | | Cal State Northridge | 75 |
| 582. | Michael Hernandez | OF | KC | 19 | 12/18/1983 | 6-0 | 175 | R-R | AA | | Daytona Beach Community College | 3 |
| 583. | Lance Dawkins | SS | CHC | 21 | 1/31/1982 | 6-3 | 190 | R-R | A- | | McNeese State University | 35 |
| 584. | Tony Neal | P | BAL | 22 | 9/12/1980 | 6-2 | 205 | R-R | A+ | | University of South Alabama | 94 |
| 585. | Brett Holmes | OF | PIT | 22 | 3/13/1981 | 6-2 | 190 | R-R | A | | Auburn University Montgomery (Ala.) | 2 |
| 586. | Micah Furtado | 2B | TEX | 21 | 6/9/1982 | 5-7 | 170 | L-R | AA | | Lewis-Clark State College | 80 |
| 587. | Scottie Anderson | SS | COL | 22 | 5/23/1981 | 5-10 | 170 | R-R | A | | Cal Poly | 64 |
| 588. | Shane Mathews | P | CLE | | | | | - | A | | St. Stephens High School (Hickory,NC) | 2 |
| 589. | Trip Mealor | P | NYM | 18 | 5/20/1985 | 6-4 | 240 | R-R | - | | Oconee County High School (Watkinsville,GA) | 1 |
| 590. | Brad Depoy | P | TOR | 18 | 9/14/1984 | 6-1 | 205 | R-R | College | | The Woodlands High School (The Woodlands,TX) | 2 |
| 591. | Dennis Dixon | OF | CIN | 18 | 1/11/1985 | 6-4 | 205 | L-R | Rookie | | San Leandro High School (San Leandro,CA) | 8 |
| 592. | J.J. Schmidt | SS | CHW | 17 | 8/15/1985 | 6-3 | 210 | R-R | Rookie | | Mira Mesa High School (San Diego,CA) | 8 |
| 593. | Salvador Encarnacion | OF | FLA | 20 | 8/26/1982 | 6-4 | 215 | R-R | Rookie | | | |
| 594. | Josh Morris | OF | BOS | 18 | 5/11/1985 | 6-5 | 230 | R-R | A | | Cartersville High School (Cartersville,GA) | 4 |
| 595. | Brad Ziegler | P | PHI | 23 | 10/10/1979 | 6-4 | 200 | R-R | MLB | 2008-2008 | Southwest Missouri State University | 64 |
| 596. | Carroll Gaddis | OF | SEA | 17 | 9/12/1985 | 6-1 | 210 | R-R | College | | Hoke County High School (Raeford,NC) | 4 |
| 597. | Terry Engles | P | MTL | 17 | 11/12/1985 | 6-4 | 170 | R-R | A- | | St. Peters Boys High School (Staten Island,NY) | 3 |
| 598. | Gregory Najac | C | MIN | 18 | 4/28/1985 | 6-0 | 210 | L-R | Rookie | | | |
| 599. | Brian Skaug | 2B | HOU | 22 | 4/13/1981 | 6-1 | 190 | R-R | A+ | | California Lutheran University | 13 |
| 600. | Jason Donald | SS | ANA | 18 | 9/4/1984 | 6-1 | 190 | R-R | AA | | Buchanan High School (Clovis,CA) | 2 |
| 601. | Chad Barben | 3B | LA | 18 | 6/15/1985 | 6-2 | 210 | R-R | - | | Taylorsville High School (Salt Lake City,UT) | 6 |
| 602. | Gordon Corder | 1B | OAK | 22 | 12/2/1980 | 6-2 | 235 | R-R | A- | | Gonzaga University | 51 |
| 603. | Raul Rodriguez | C | SF | 18 | 7/27/1984 | 6-0 | 195 | R-R | - | | Florida Christian High School (Miami,FL) | 3 |
| 604. | Daniel Bard | P | NYY | 18 | 6/25/1985 | 6-4 | 185 | R-R | AA | | Charlotte Christian High School (Charlotte,NC) | 2 |
| 605. | Jordan Pals | P | STL | 22 | 10/18/1980 | 6-8 | 205 | R-R | AA | | Eastern Illinois University | 58 |
| 606. | Joe Carque | P | ARI | 22 | 5/20/1981 | 6-2 | 185 | R-R | AAA | | San Diego State University | 155 |
| 607. | Kyle Bakker | P | ATL | 21 | 10/21/1981 | 6-9 | 250 | L-L | A | | Georgia Tech | 109 |
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