| 1 | 18 | June-Reg | Royce Ring | P | 21 | 6-5 | 220 | L | L | MLB | 2005-2008 | San Diego State University | |
| 2 | 59 | June-Reg | Jeremy Reed | OF | 21 | 6-0 | 160 | L | L | MLB | 2004-2009 | Long Beach State University | $650,000 |
| 3 | 90 | June-Reg | Josh Rupe | P | 19 | 6-2 | 180 | R | R | MLB | 2005-2009 | Louisburg College | $440,000 |
| 4 | 120 | June-Reg | Ryan Rodriguez | P | 17 | 6-4 | 210 | L | L | AAA | | Keller (Keller,TX) | |
| 5 | 150 | June-Reg | B.J. LaMura | P | 21 | 6-1 | 200 | R | R | AAA | | Clemson University | |
| 6 | 180** | June-Reg | Chris Getz | SS | 18 | 6-0 | 165 | L | R | MLB | 2008-2009 | Grosse Point South (Grosse Pointe,MI) | |
| 7 | 210 | June-Reg | Micah Schnurstein | 3B | 17 | 6-1 | 200 | R | R | AA | | Basic (Henderson,NV) | |
| 8 | 240 | June-Reg | Sean Tracey | P | 21 | 6-3 | 210 | L | R | MLB | 2006-2006 | University of California-Irvine | |
| 9 | 270 | June-Reg | Todd Deininger | P | 20 | 6-3 | 200 | R | R | A | | Texas A&M University | |
| 10 | 300 | June-Reg | Orionny Lopez | P | 18 | 6-2 | 170 | R | R | AAA | | Forest Hill (West Palm Beach,FL) | |
| 11 | 330 | June-Reg | Matt Herring | 1B | 21 | 6-2 | 210 | L | L | Rookie | | Georgia Southern University | |
| 12 | 360 | June-Reg | Jayson Ruhlman | P | 17 | 6-1 | 180 | L | L | AAA | | L'anse Creuse North (Chesterfield,MI) | |
| 13 | 390 | June-Reg | Demetrius Banks | P | 19 | 6-1 | 160 | L | L | A+ | | Chattahoochee Valley Community College | |
| 14 | 420 | June-Reg | Christian Madson | P | 18 | 6-8 | 250 | R | R | College | | Bloomingdale (Valrico,FL) | |
| 15 | 450 | June-Reg | Adam Larson | P | 22 | 6-3 | 230 | R | R | AAA | | Middle Tennessee State University | |
| 16 | 480 | June-Reg | Daniel Haigwood | P | 18 | 6-2 | 200 | R | L | AAA | | Midland (Pleasant Plains,AR) | |
| 17 | 510 | June-Reg | Brandon McCarthy | P | 18 | 6-7 | 180 | R | R | MLB | 2005-2009 | Lamar Community College | $40,000 |
| 18 | 540 | June-Reg | Shane Scoville | C | 22 | 6-0 | 180 | R | R | Rookie | | University of South Alabama | |
| 19 | 570** | June-Reg | Paul Keck | C | 18 | 6-3 | 205 | R | R | Rookie | | Granada Hills (Granada Hills,CA) | |
| 20 | 600 | June-Reg | Boone Logan | P | 17 | 6-5 | 200 | R | L | MLB | 2006-2009 | Sandra Day O'Connor (Helotes,TX) | |
| 21 | 630 | June-Reg | Seth Morris | OF | 21 | 6-2 | 200 | R | R | A | | University of Kentucky | |
| 22 | 660 | June-Reg | Eric Keefner | 1B | 20 | 6-3 | 220 | R | R | Ind | | Mesa Community College | |
| 23 | 690 | June-Reg | David Beasley | P | | | 0 | | | - | | Cooper City (Cooper City,FL) | |
| 24 | 720 | June-Reg | Thomas Brice | OF | 20 | 6-5 | 210 | L | L | A+ | | Faulkner State Community College | |
| 25 | 750 | June-Reg | Jay Marshall | P | 19 | 6-5 | 185 | L | L | MLB | 2007-2009 | Jefferson College | |
| 26 | 780** | June-Reg | Kris Dufner | SS | 22 | 6-2 | 190 | B | R | A+ | | University of Delaware | |
| 27 | 810 | June-Reg | Michael Bohlander | 1B | 21 | 6-1 | 230 | L | R | Rookie | | Pace University | |
| 28 | 840** | June-Reg | Hector Ambriz | P-C | 18 | 6-1 | 220 | L | R | AAA | | Valencia (Placentia,CA) | |
| 29 | 870** | June-Reg | Neil Giesler | 1B | 18 | 6-5 | 175 | L | R | College | | Jasper (Jasper,IN) | |
| 30 | 900 | June-Reg | Jake Nowlen | P | 21 | 6-2 | 190 | R | R | Rookie | | University of Arkansas | |
| 31 | 930 | June-Reg | Edgar Varela | 3B | 21 | 6-1 | 200 | L | R | A+ | | Long Beach State University | |
| 32 | 960 | June-Reg | Rick Hummel | P | 21 | 6-2 | 190 | R | R | A+ | | University of Indianapolis | |
| 33 | 990** | June-Reg | Tony Sipp | P | 18 | 6-0 | 185 | L | L | MLB | 2009-2009 | Northwest Florida State College | |
| 34 | 1020** | June-Reg | Brandon Durden | P | 17 | 6-3 | 215 | R | L | AA | | Cook (Adel,GA) | |
| 35 | 1050** | June-Reg | Eric Everly | P | 18 | 6-3 | 215 | L | L | Rookie | | Seagoville (Seagoville,TX) | |
| 36 | 1080 | June-Reg | Jason Pilkington | P | 21 | 6-6 | 205 | R | L | Ind | | Central Arizona College | |
| 37 | 1110 | June-Reg | Jamin Huchingson | P | 18 | 6-2 | 189 | R | R | Rookie | | Fayetteville (Fayetteville,AR) | |
| 38 | 1140** | June-Reg | D.J. Wabick | LF | 18 | 6-2 | 185 | L | R | AA | | Stagg (Palos Hills,IL) | |
| 39 | 1170 | June-Reg | Gabriel Casanova | 2B | 17 | 6-1 | 190 | R | R | Rookie | | Coral Park (Miami,FL) | |
| 40 | 1200 | June-Reg | Dennis Pawelek | P | 21 | 6-3 | 190 | R | R | - | | Snow College | |
| 41 | 1230** | June-Reg | Daniel Barone | P | 19 | 6-2 | 185 | R | R | MLB | 2007-2007 | Monterey Peninsula College | |
| 42 | 1260 | June-Reg | Ian Ganzer | P | | | 0 | | | - | | Wellington Christian (Wellington,FL) | |
| 43 | 1289 | June-Reg | Matthew Sibigtroth | SS | | | 0 | | | College | | Kishwaukee College | |
| 44 | 1317 | June-Reg | Ramon Castro | 2B-SS | | | 0 | | | - | | George Washington (New York,NY) | |
| 45 | 1344 | June-Reg | Jorge Mico | C | 17 | 5-11 | 195 | R | R | College | | Hialeah (Hialeah,FL) | |
| 46 | 1371 | June-Reg | Jeremy Paul | C | | | 0 | | | Ind | | Trinity International (Trinity International) | |
| 47 | 1397** | June-Reg | Tim Grogan | | 18 | 6-0 | 195 | L | R | A- | | Covington Catholic (Park Hills,KY) | |
| 48 | 1423** | June-Reg | Anthony Manuel | 2B | | | 0 | | | A | - | Kishwaukee College | |
| 49 | 1448 | June-Reg | Fernando Hernandez | P | 17 | 5-11 | 190 | R | R | MLB | 2008-2008 | Southwest (Miami,FL) | |
| 50 | 1472 | June-Reg | Matt Payne | P | 23 | 6-2 | 190 | R | R | Rookie | | University of California | |