| 1 | 25 | June-Reg | Matt Cain | P | 17 | 6-3 | 180 | R | R | MLB | 2005-2009 | Houston (Germantown,TN) | $1,375,000 |
| 2 | 66 | June-Reg | Fred Lewis | OF | 21 | 6-2 | 190 | L | R | MLB | 2006-2009 | Southern University | $595,000 |
| 3 | 97 | June-Reg | Daniel Ortmeier | OF | 21 | 6-4 | 225 | R | L | MLB | 2005-2008 | University of Texas | |
| 4 | 127 | June-Reg | Kevin Correia | P | 21 | 6-3 | 180 | R | R | MLB | 2003-2009 | Cal Poly | $105,000 |
| 5 | 157 | June-Reg | Kevin Kelly | SS | 22 | 6-1 | 200 | R | R | A | | Duke University | |
| 6 | 187 | June-Reg | Jesse English | P | 17 | 6-3 | 22 | L | L | AA | | Rancho Buena Vista (Vista,CA) | |
| 7 | 217 | June-Reg | Mike Musgrave | P | 18 | 6-2 | 185 | R | R | AAA | | Ocala (Ocala,FL) | |
| 8 | 247 | June-Reg | Clay Hensley | P | 22 | 5-11 | 175 | R | R | MLB | 2005-2008 | Lamar University | |
| 9 | 277 | June-Reg | Randy Walter | OF | 21 | 6-2 | 200 | R | R | AA | | Wichita State University | |
| 10 | 307 | June-Reg | Glenn Woolard | P | 21 | 6-1 | 200 | R | R | AAA | | Kutztown University | |
| 11 | 337 | June-Reg | Jake Wald | 3B | 21 | 6-2 | 180 | R | R | AAA | | George Washington University | |
| 12 | 367** | June-Reg | Kellen Ludwig | P | 19 | 6-4 | 230 | R | R | A- | | Chipola College | |
| 13 | 397 | June-Reg | Aaron Sobieraj | 2B | 21 | 6-1 | 192 | R | R | A+ | | University of Florida | |
| 14 | 427 | June-Reg | Josh Habel | P | 21 | 6-1 | 185 | L | L | AA | | University of Northern Iowa | |
| 15 | 457 | June-Reg | Matthew Dryer | 3B | 22 | 6-2 | 190 | R | R | AA | | University of Miami | |
| 16 | 487 | June-Reg | Greg Bruso | P | 22 | 6-3 | 190 | R | R | AA | | University of California | |
| 17 | 517 | June-Reg | Luke Nelson | P | 22 | 6-2 | 180 | R | R | A- | | Southern Illinois University | |
| 18 | 547 | June-Reg | Bryan Millikan | P | 18 | 6-5 | 190 | R | R | AA | | Black Hills (Olympia,WA) | |
| 19 | 577 | June-Reg | David Timm | P | 18 | 6-6 | 190 | L | L | A | | Seminole Community College | |
| 20 | 607 | June-Reg | Benjamin Mitchell | P | 22 | 6-3 | 205 | R | R | A- | | Grand Canyon University | |
| 21 | 637 | June-Reg | Travis Ishikawa | 1B | 18 | 6-3 | 190 | L | L | MLB | 2006-2009 | Federal Way (Federal Way,WA) | $955,000 |
| 22 | 667 | June-Reg | Nelson Lopez | P | 21 | 6-2 | 205 | R | R | A | | Florida Atlantic University | |
| 23 | 697** | June-Reg | Neil Walton | H | 18 | 6-5 | 180 | R | R | AA | | Santa Cruz (Santa Cruz,CA) | |
| 24 | 727 | June-Reg | Jay Knowlton | SS | 22 | 6-1 | 200 | R | R | AA | | Lewis and Clark College | |
| 25 | 757 | June-Reg | Justin Gee | P | 18 | 6-4 | 200 | R | R | - | | Sarasota (Sarasota,FL) | |
| 26 | 787** | June-Reg | Sean Rierson | P | 21 | 6-3 | 197 | R | R | College | | University of Arizona | |
| 27 | 817 | June-Reg | Ryan McGovern | P | 18 | 6-1 | 185 | L | L | Rookie | | WJ Mouat SS (Abbotsford,Can) | |
| 28 | 847 | June-Reg | Phillip Tapley | P | | | 0 | | | - | | Greenbrier (Evans,GA) | |
| 29 | 877** | June-Reg | Alex Hinshaw | P | 19 | 6-3 | 170 | L | L | MLB | 2008-2009 | Chaffey College | |
| 30 | 907 | June-Reg | Matthew Somnis | P | | | 0 | | | - | | Lassen Community College | |
| 31 | 937 | June-Reg | Matt Palmer | P | 23 | 6-2 | 205 | R | R | MLB | 2008-2009 | Missouri State University | |
| 33 | 997** | June-Reg | Brett Cooley | 1B | 21 | 6-4 | 220 | R | R | Ind | | University of Houston | |
| 34 | 1027 | June-Reg | Phil Monte | P | | | 0 | | | Ind | | Cosumnes River College | |
| 35 | 1057 | June-Reg | Ryan Stevenson | CF | 19 | 6-1 | 185 | L | L | Ind | | Chabot College | |
| 36 | 1087 | June-Reg | David Stone | OF | 22 | 6-2 | 185 | L | R | A- | | University of Virginia | |
| 37 | 1117 | June-Reg | Chris Sweet | | 21 | 6-1 | 185 | R | R | College | | University of Virginia | |
| 38 | 1147 | June-Reg | Randal Hodge | P | | | 0 | | | - | | Pearl-Cohn (Nashville,TN) | |
| 39 | 1177 | June-Reg | Anthony Moreno | P | 18 | 6-1 | 190 | R | R | AA | | South Mountain Community College | |
| 40 | 1207 | June-Reg | Mike Redford | P | 18 | | 0 | | | - | | Woodland (Woodland,CA) | |
| 41 | 1237** | June-Reg | Danny Muegge | | 21 | 6-5 | 175 | L | R | AA | | University of Texas | |
| 42 | 1267 | June-Reg | Drew Jefferson | P | 22 | 5-9 | 185 | L | L | A- | | Missouri State University | |
| 43 | 1296 | June-Reg | Jason Chavez | P | 19 | 6-5 | 195 | R | R | College | | Mineral Area College | |
| 44 | 1324 | June-Reg | Tim Torres | SS | 18 | 6-2 | 180 | B | R | AA | | Diablo Valley College | |
| 45 | 1351** | June-Reg | Matt Berezay | OF | 18 | 5-11 | 190 | B | R | AA | | Sierra Vista (Baldwin Park,CA) | |
| 46 | 1378 | June-Reg | Matt Ircandia | P | 17 | 6-3 | 200 | L | L | College | | Semiahmoo Secondary School (Surrey,BC) | |
| 47 | 1404 | June-Reg | Scott Dodge | H | | | | L | | College | | Cuesta College | |
| 48 | 1430** | June-Reg | Dante Brinkley | OF | 20 | 5-10 | 170 | R | R | AAA | | Missouri State University | |
| 49 | 1455** | June-Reg | Zach Borowiak | SS | 21 | 6-1 | 185 | R | R | AAA | | Southeast Missouri State University | |
| 50 | 1479** | June-Reg | Robert Davis | C | 18 | 6-0 | 195 | R | R | A- | | Greenbrier (Evans,GA) | |
| 32 | 4508 | June-Reg | Joe Bateman | P | 22 | 6-2 | 175 | R | R | AAA | | University of Massachusetts | |
