| 1 | 1 | June-Reg | Joe Mauer | C | 18 | 6-4 | 220 | L | R | MLB | 2004-2009 | Cretin-Derham Hall (St. Paul,MN) | $4,000,000 |
| 2 | 45 | June-Reg | Scott Tyler | P | 18 | 6-5 | 210 | R | R | AA | | Downington (Downington,PA) | $800,000 |
| 3 | 77 | June-Reg | Jose Morales | SS | 18 | 5-11 | 170 | B | R | MLB | 2007-2009 | Academia de Providencia (Rio Piedras,PR) | $450,000 |
| 4 | 107 | June-Reg | Angel Garcia | P | 17 | 6-6 | 200 | R | R | AA | | Nicolas Sevilla (Toa Alta,PR) | $310,000 |
| 5 | 137 | June-Reg | Jeremy Brown | P | 22 | 6-3 | 210 | R | R | Rookie | | University of Georgia | $197,500 |
| 6 | 167 | June-Reg | Vince Serafini | P | 21 | 6-1 | 230 | R | L | A | | University of Evansville | $140,000 |
| 7 | 197 | June-Reg | Matt Vorwald | P | 21 | 6-2 | 186 | B | R | A | | University of Illinois | $117,500 |
| 8 | 227 | June-Reg | Jared Hemus | P | 20 | 5-11 | 168 | L | L | A | | Grossmont College | $80,000 |
| 9 | 257 | June-Reg | Dusty Gomon | 1B | 18 | 6-3 | 190 | R | R | A+ | | Terry Parker (Jacksonville,FL) | $70,000 |
| 10 | 287 | June-Reg | Garrett Guzman | OF | 18 | 5-10 | 170 | L | L | AAA | | Green Valley (Henderson,NV) | $75,000 |
| 11 | 317 | June-Reg | Josh Renick | 2B | 22 | 5-9 | 180 | R | R | AA | | Middle Tennessee State University | |
| 12 | 347 | June-Reg | Kaulana Kuhaulua | IF | 21 | 6-0 | 166 | R | R | AA | | Long Beach State University | |
| 13 | 377 | June-Reg | Kevin Cameron | P | 21 | 6-1 | 181 | R | R | MLB | 2007-2009 | Georgia Tech | |
| 14 | 407** | June-Reg | Ryan Anderson | P | 18 | 6-1 | 180 | L | L | - | | Gaither (Tampa,FL) | |
| 15 | 437 | June-Reg | Brett Lawson | P | 22 | 6-7 | 230 | R | R | A | | Northwestern Oklahoma State University | |
| 16 | 467** | June-Reg | Brian Stitt | P | 18 | 6-0 | 180 | R | R | AAA | | Wellington Christian (Wellington,FL) | |
| 17 | 497** | June-Reg | Matt Macri | SS | 19 | 6-2 | 200 | R | R | MLB | 2008-2008 | Dowling (West Des Moines,IA) | |
| 18 | 527 | June-Reg | Robert Guzman | OF | 21 | 5-11 | 200 | L | L | A | | Cal State Fullerton | |
| 19 | 557 | June-Reg | Scott Whitrock | OF | 20 | 6-0 | 180 | R | R | AA | | Madison Area Technical College | |
| 20 | 587 | June-Reg | Anthony Albano | OF | 18 | 6-4 | 200 | L | R | Ind | | Brother Rice (Chicago,IL) | |
| 21 | 617 | June-Reg | Felix Molina | 2B | 18 | 5-7 | 160 | B | R | AAA | | Eugenio Maria De Hostos (Mayaguez,PR) | |
| 22 | 647 | June-Reg | Ryan Smith | C | 20 | 6-1 | 210 | B | R | A | | Citrus College | |
| 23 | 677 | June-Reg | Jake Mauer | 2B | 22 | 6-2 | 180 | R | R | AA | | Saint Thomas (Mendota Heights,MN) | |
| 24 | 707 | June-Reg | Bryan Kennedy | C | 22 | 6-2 | 208 | L | R | AAA | | University of California-Riverside | |
| 25 | 737 | June-Reg | Josh Johnson | P | 18 | 5-10 | 180 | R | R | AAA | | Ridgway (Ridgway,PA) | |
| 26 | 767** | June-Reg | John Herrera | | 18 | 6-4 | 200 | R | R | A+ | | Redlands (Redlands,CA) | |
| 27 | 797 | June-Reg | Justin Elliott | OF | 19 | 5-11 | 180 | R | R | AAA | | Lake Gibson (Lakeland,FL) | |
| 28 | 827 | June-Reg | Brian Gates | P | 21 | 6-0 | 195 | R | R | A | | University of Tennessee | |
| 29 | 857 | June-Reg | Nick Blackburn | P | 19 | 6-4 | 230 | R | R | MLB | 2007-2009 | Seminole State College | |
| 30 | 887 | June-Reg | Josh Daws | P | 22 | 5-10 | 192 | R | R | A+ | | Jacksonville University | |
| 31 | 917** | June-Reg | Ben Thomas | 3B | 19 | 6-0 | 190 | L | R | A+ | | Rapid City Central (Rapid City,SD) | |
| 32 | 947 | June-Reg | Jason Wilmes | P | 18 | 6-6 | 215 | L | L | - | | Somerset (Somerset,WI) | |
| 33 | 977 | June-Reg | Ryan Gehring | P | 19 | 6-4 | 205 | L | L | Ind | | Burlington Catholic Center (Burlington,WI) | |
| 34 | 1007 | June-Reg | Marshall Hendon | | | | | | | College | | Christian Brothers (Sacramento,CA) | |
| 35 | 1037** | June-Reg | Bookie Gates | 2B | 21 | 6-2 | 200 | R | R | A- | | Washington State University | |
| 36 | 1067 | June-Reg | Kenny Huff | 1B | 21 | 5-11 | 211 | L | L | A | - | University of Arizona | |
| 37 | 1097 | June-Reg | Shawn Tarkington | P | 21 | 6-4 | 230 | R | R | Rookie | | Seton Hall University | |
| 38 | 1127 | June-Reg | Matt Abram | OF | 21 | 6-0 | 174 | R | R | A+ | | University of Arizona | |
| 39 | 1157 | June-Reg | Nick Niedbalski | P | 20 | 6-2 | 190 | R | L | Ind | | East Central Community College | |
| 40 | 1187 | June-Reg | Erik Lohse | P | 21 | 6-1 | 205 | R | R | AA | | Sacramento City College | |
| 41 | 1216 | June-Reg | Ryan Spataro | OF | 18 | 5-10 | 180 | L | R | A | | St. Peter's Secondary School (Barrie,ON) | |
| 42 | 1245 | June-Reg | Patrick Tingley | P | 20 | 6-2 | 210 | L | L | Ind | | Indiana State University | |
| 43 | 1273** | June-Reg | Jason Vargas | | 18 | 6-0 | 215 | L | L | MLB | 2005-2009 | | |
| 44 | 1301 | June-Reg | Ryan Hader | P | 18 | 6-4 | 240 | R | L | Ind | | Belton (Belton,MO) | |
| 45 | 1329 | June-Reg | Joshua Smith | RF | | | 0 | | | - | | Canyon Springs (Moreno Valley,CA) | |
| 46 | 1357 | June-Reg | Mitch Pruemer | | | | | | | College | | Rend Lake (Dieterich,IL) | |
| 47 | 1385 | June-Reg | Daniel Smith | RF | | | 0 | | | - | | Eau Gallie (Melbourne,FL) | |
| 48 | 1412 | June-Reg | William Guzman | SS | 17 | | 0 | R | R | - | | Northwest Christian (Juana Diaz,PR) | |
| 49 | 1438 | June-Reg | Jason Paul | | | | | | | College | | Ball State University | |
| 50 | 1463 | June-Reg | Robert Strickland | 2B | | | 0 | | | - | | Frostproof (Frostproof,FL) | |