| Darwin Alvarado | of | 18.243 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Brian Anderson | SS | 24.053 | Miami Marlins | Jacksonville (AA) | Reinstated to active roster |
| Keyder Aristigueta | 2b | 21.160 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Gio Arriera | p | 19.034 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Jheyser Azuaje | c | 20.149 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Jake Bivens | if | 22.047 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Ulrich Bojarski | of | 18.299 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Brandon Boone | p | 22.258 | Miami Marlins | Batavia (A-) | Assigned to Batavia (A-) |
| Daniel Calderon | p | 19.271 | New York Yankees | Gulf Coast Yankees (Rk) | Assigned to Gulf Coast Yankees (Rk) |
| Drew Carlton | p | 21.307 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Shane Cooper | ss | 22.204 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Drew Crosby | p | 21.238 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Bobby Dalbec | 3b | 22.012 | Boston Red Sox | Greenville (A) | Assigned to Greenville (A) |
| Isrrael De la Cruz | ss | 20.026 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Francisco Diaz | C | 27.112 | New York Yankees | Staten Island (A-) | Activated from disabled list |
| Logan Farrar | of | 22.086 | Oakland Athletics | Vermont (A-) | Assigned to Vermont (A-) |
| Aaron Fernandez | p | 22.289 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Ken Figueroa | p | 21.042 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Alexis Garcia | ss | 20.010 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Joe Gomez | c | 22.220 | St. Louis Cardinals | State College (A-) | Assigned to State College (A-) |
| Nick Gordon | SS | 21.261 | Minnesota Twins | Chattanooga (AA) | Reinstated to active roster |
| Derek Hill | OF | 21.194 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Xavier Javier | p | 19.152 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Ryan Karstetter | 3b | 20.162 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Billy Lescher | p | 21.298 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Chris Mazza | P | 27.267 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Sam McMillan | c | 18.222 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Cameron Ming | p | 21.070 | Baltimore Orioles | Aberdeen (A-) | Assigned to Aberdeen (A-) |
| Tanner Nishioka | 3b | 22.262 | Boston Red Sox | Lowell (A-) | Assigned to Lowell (A-) |
| Durin O'Linger | p | 23.274 | Boston Red Sox | Lowell (A-) | Assigned to Lowell (A-) |
| Jordan Pearce | 3b | 21.027 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Dillon Peters | P | 24.314 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Blake Pflughaupt | p | 20.310 | Tampa Bay Rays | Hudson Valley (A-) | Assigned to Hudson Valley (A-) |
| Jose Polonia | 3b | 21.213 | New York Yankees | Staten Island (A-) | Assigned to Staten Island (A-) |
| Grant Reuss | p | 21.049 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Josh Roeder | p | 24.221 | New York Yankees | Staten Island (A-) | Assigned to Staten Island (A-) |
| Max Ryan | p | 20.033 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Jose Salas | ss | 20.085 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Kit Scheetz | p | 23.054 | Houston Astros | Tri City (A-) | Assigned to Tri City (A-) |
| Mitch Stalsberg | p | 21.180 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Dylan Stock | p | 20.355 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Josh Taylor | P | 24.131 | Arizona Diamondbacks | Jackson (AA) | Assigned to Jackson (AA) |
| Kyle Thomas | p | 22.128 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Ignacio Valdez | of | 21.361 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Javier Villarroel | p | 19.253 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Felix Viloria | p | 20.221 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to Gulf Coast Blue Jays (Rk) |
| Nolan Watson | p | 20.167 | Kansas City Royals | Lexington (A) | Assigned to Lexington (A) |
| Rob Zastryzny | RP | 25.107 | Chicago Cubs | Iowa (AAA) | Activated from disabled list |
| Todd Cunningham | LF | 28.113 | Los Angeles Dodgers | Los Angeles Dodgers (MLB) | Traded from Cardinals to Dodgers for cash considerations |
| Kolten Wong | 2B | 26.274 | St. Louis Cardinals | St. Louis Cardinals (MLB) | Activated from DL |
| David Washington | 1B | 26.233 | Baltimore Orioles | Norfolk (AAA) | Outrighted to minors |
| Paul Janish | SS | 34.272 | Baltimore Orioles | Baltimore Orioles (MLB) | Outrighted to minors |
| Cameron Seitzer | 1B | 27.181 | Chicago White Sox | Birmingham (AA) | DL-7 Injured |
| James Needy | P | 26.103 | Miami Marlins | Jupiter (A+) | DL-7 Injured |
| Sean O'Connell | P | 25.212 | Los Angeles Dodgers | Tulsa (AA) | DL-7 Injured |
| Tim Shibuya | P | 27.300 | Los Angeles Dodgers | Tulsa (AA) | DL-7 Injured |
| Zach Jones | C-LF | 26.219 | Minnesota Twins | Gulf Coast Twins (Rk) | DL-7 Injured |
| Ashton Goudeau | P | 24.353 | Kansas City Royals | Northwest Arkansas (AA) | DL-7 Injured |
| Blake McKnight | P | 26.148 | | | Released by Cardinals |
| Christopher Kirsch | P | 25.239 | Tampa Bay Rays | Montgomery (AA) | DL-7 Injured |
| Claudio Bautista | 3b | 23.224 | Cleveland Indians | Lynchburg (A+) | DL-7 Injured |
| Drew Ferguson | of | 24.342 | Houston Astros | Corpus Christi (AA) | DL-7 Injured |
| Wynston Sawyer | C | 25.240 | Los Angeles Dodgers | Tulsa (AA) | DL-7 Injured |
| Brandon Diaz | CF | 22.088 | Milwaukee Brewers | Carolina (A+) | DL-7 Injured |
| Francis Christy | c | 21.314 | Arizona Diamondbacks | Kane County (A) | DL-7 Injured |
| Gabriel Valdez | p | 21.260 | Houston Astros | Quad Cities (A) | Placed on temporary inactive list |
| Mark Armstrong | P | 22.227 | Cincinnati Reds | Daytona (A+) | DL-7 Injured |
| Yusniel Diaz | of | 20.277 | Los Angeles Dodgers | Rancho Cucamonga (A+) | DL-7 Injured |
| Braden Webb | p | 22.077 | Milwaukee Brewers | Wisconsin (A) | DL-7 Injured |
| Erling Moreno | p | 20.179 | Chicago Cubs | South Bend (A) | DL-7 Injured |
| Patrick Riehl | p | 23.053 | Cincinnati Reds | Dayton (A) | DL-7 Injured |
| Sam McWilliams | P | 21.311 | Arizona Diamondbacks | Kane County (A) | DL-7 Injured |
| Thomas Jankins | p | 22.009 | Milwaukee Brewers | Wisconsin (A) | DL-7 Injured |
| Yoel Vasquez | c | 20.325 | Milwaukee Brewers | Wisconsin (A) | |
| Kenny Mendoza | p | 19.121 | Texas Rangers | Arizona Rangers (Rk) | Suspended |
| Jose Lizarraga | c | 19.318 | Baltimore Orioles | Dominican Orioles (Rk) | Assigned to DSL Orioles (Rk) |
| Vincent Velasquez | SP | 25.034 | Philadelphia Phillies | Philadelphia Phillies (MLB) | Recalled from rehab assignment |
| Alex Faedo | p | 21.242 | Detroit Tigers | Gulf Coast Tigers West (Rk) | Signed out of draft (Florida) |
| Dakota Bennett | p | 17.365 | Miami Marlins | Gulf Coast Marlins (Rk) | Signed out of draft (Alabama HS) |
| David Metzgar | 2b | 22.213 | New York Yankees | Gulf Coast Yankees West (Rk) | Assigned to GCL Yankees West (Rk) |
| Michael Smith | of | 20.042 | Tampa Bay Rays | Gulf Coast Rays (Rk) | Signed out of draft (San Jacinto) |
| Peter Solomon | p | 20.329 | Houston Astros | Gulf Coast Astros (Rk) | Signed out of draft (Notre Dame) |
| Tyler Zombro | p | 24.312 | Tampa Bay Rays | Gulf Coast Rays (Rk) | Signed as undrafted free agent (George Mason) |
| Yasmany Tomas | LF | 26.239 | Arizona Diamondbacks | Arizona Diamondbacks (MLB) | Recalled from rehab assignment |
