| Justin Upton | LF | 28.327 | Detroit Tigers | Detroit Tigers (MLB) | Reinstated from bereavement list |
| Zach Davies | SP | 23.160 | Milwaukee Brewers | Milwaukee Brewers (MLB) | Recalled from Colorado Springs (AAA) |
| Jacob Turner | RP | 25.057 | Chicago White Sox | Chicago White Sox (MLB) | Contract purchased from minors |
| Eric O'Flaherty | RP | 31.162 | Atlanta Braves | Atlanta Braves (MLB) | Activated from DL |
| Arodys Vizcaino | RP | 25.246 | Atlanta Braves | Atlanta Braves (MLB) | DL-15 Strained right oblique |
| Gerrit Cole | SP | 25.312 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | Activated from DL |
| Trayce Thompson | LF-RF | 25.124 | Los Angeles Dodgers | Los Angeles Dodgers (MLB) | DL-15 Lower back irritation |
| Zach Walters | SS | 26.315 | Los Angeles Dodgers | Los Angeles Dodgers (MLB) | Recalled from Oklahoma City (AAA) |
| Wade Davis | CL | 30.313 | Kansas City Royals | Kansas City Royals (MLB) | Activated from DL |
| Matt Duffy | 3B | 27.161 | Houston Astros | Fresno (AAA) | Designated for Assignment |
| Justin Smoak | 1B | 29.224 | Toronto Blue Jays | Toronto Blue Jays (MLB) | Signed to 2-year contract for 2017-2018 ($8.25m) Club option for 2019 @$6m 4.125--4.125 |
| Yu Darvish | SP | 29.335 | Texas Rangers | Texas Rangers (MLB) | Activated from DL |
| Yhoan Acosta | P | 21.030 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Sergio Alcantara | SS | 20.007 | Arizona Diamondbacks | Kane County (A) | Assigned to Kane County (A) |
| Isaac Anderson | p | 22.316 | Los Angeles Dodgers | Rancho Cucamonga (A+) | Assigned to Rancho Cucamonga (A+) |
| Bryan Araiza | of | 20.081 | Arizona Diamondbacks | Missoula (Rk) | Assigned to Missoula (Rk) |
| Manny Argomaniz | c | 23.104 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Junior Arias | P | 22.249 | Cincinnati Reds | Billings (Rk) | Assigned to Billings (Rk) |
| Pedro Avila | p | 19.184 | Washington Nationals | Hagerstown (A) | Reinstated to active roster |
| Julian Aybar | p | 24.155 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Anthony Banda | P | 22.341 | Arizona Diamondbacks | Reno (AAA) | Reinstated to active roster |
| Andrew Barbosa | P | 28.242 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Buddy Baumann | P | 28.221 | San Diego Padres | El Paso (AAA) | Assigned to El Paso (AAA) |
| Gerson Bautista | p | 21.047 | Boston Red Sox | Greenville (A) | Assigned to Greenville (A) |
| Reiny Beltre | 3b | 20.001 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Anthony Bemboom | C | 26.180 | Los Angeles Angels | Arkansas (AA) | Assigned to Arkansas (AA) |
| Tim Berry | P | 25.121 | San Diego Padres | San Antonio (AA) | Assigned to San Antonio (AA) |
| Alex Bisacca | p | 23.024 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| John Brebbia | P | 26.048 | St. Louis Cardinals | Springfield (AA) | Assigned to Springfield (AA) |
| Braden Bristo | p | 21.258 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Matt Buschmann | RP | 32.155 | Arizona Diamondbacks | Reno (AAA) | Activated from disabled list |
| Alex Call | of | 21.293 | Chicago White Sox | Kannapolis (A) | Assigned to Kannapolis (A) |
| Miles Chambers | p | 22.268 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Adam Cimber | P | 25.336 | San Diego Padres | San Antonio (AA) | Assigned to San Antonio (AA) |
| Jonathon Crawford | P | 24.259 | Cincinnati Reds | Arizona Reds (Rk) | Assigned to Arizona Reds (Rk) |
| Galli Cribbs | IF | 23.282 | Arizona Diamondbacks | Visalia (A+) | Assigned to Visalia (A+) |
| William Cuevas | P | 25.276 | Boston Red Sox | Pawtucket (AAA) | Assigned to Pawtucket (AAA) |
| Luis De Paula | P | 24.082 | Los Angeles Dodgers | Rancho Cucamonga (A+) | Activated from disabled list |
| Jeff Degano | p | 23.260 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Ross Detwiler | RP | 30.133 | Oakland Athletics | Nashville (AAA) | Traded from Indians to Athletics for cash considerations |
| Andy Diaz | of | 20.239 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Yusniel Diaz | of | 19.283 | Los Angeles Dodgers | Rancho Cucamonga (A+) | Assigned to Rancho Cucamonga (A+) |
| Phillip Diehl | p | 22.001 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Abel Duarte | p | 22.058 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Jacob Evans | p | 22.232 | St. Louis Cardinals | Palm Beach (A+) | Activated from disabled list |
| Andy Ferguson | P | 27.318 | Kansas City Royals | Omaha (AAA) | Assigned to Omaha (AAA) |
| Icezack Flemming | p | 24.023 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Junior Flores | p | 21.277 | Milwaukee Brewers | Wisconsin (A) | Assigned to Wisconsin (A) |
| Estevan Florial | of | 18.234 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Mike Ford | P | 24.013 | New York Yankees | Tampa (A+) | Assigned to Tampa (A+) |
| Wander Franco | 3b | 19.279 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Dermis Garcia | ss | 18.191 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Wilkerman Garcia | ss | 18.107 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Isiah Gilliam | of | 19.359 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Zack Hartman | p | 24.198 | Los Angeles Angels | Inland Empire (A+) | Assigned to Inland Empire (A+) |
| Wes Helsabeck | p | 24.010 | Los Angeles Dodgers | Great Lakes (A) | Assigned to Great Lakes (A) |
| Harley Holt | p | 24.263 | St. Louis Cardinals | State College (A-) | Assigned to State College (A-) |
| Tyler Honahan | p | 22.193 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Garrett Hope | c | 22.202 | Los Angeles Dodgers | Great Lakes (A) | Activated from disabled list |
| Luis Jean | SS | 21.334 | Colorado Rockies | Modesto (A+) | Activated from disabled list |
| Hunter Jones | OF | 24.335 | Chicago White Sox | Birmingham (AA) | Assigned to Birmingham (AA) |
| Will Jones | p | 23.124 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Kevin Josephina | 2b | 19.288 | Atlanta Braves | Rome (A) | Assigned to Rome (A) |
| Michael Kelly | P | 23.314 | San Diego Padres | El Paso (AAA) | Assigned to El Paso (AAA) |
| Brady Lail | P | 22.342 | New York Yankees | Trenton (AA) | Activated from disabled list |
| John Lamb | SP | 26.007 | Cincinnati Reds | Louisville (AAA) | Assigned to Louisville (AAA) |
| Rafael Lara | p | 21.037 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Landon Lassiter | of | 23.033 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Jack Leathersich | RP | 26.003 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Tim Lynch | 1b | 23.044 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Justin Maese | p | 19.266 | Toronto Blue Jays | Lansing (A) | Assigned to Lansing (A) |
| Marc Magliaro | P | 26.150 | Colorado Rockies | Modesto (A+) | Assigned to Modesto (A+) |
| Spencer Mahoney | p | 23.037 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Pedro Marquez | of | 22.179 | Arizona Diamondbacks | Missoula (Rk) | Assigned to Missoula (Rk) |
| Chad Martin | p | 22.196 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Robert Martinez | OF | 22.159 | Oakland Athletics | Beloit (A) | Assigned to Beloit (A) |
| Welfrin Mateo | 2b | 20.313 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Leonardo Molina | OF | 19.004 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Chan Moon | SS | 25.116 | Houston Astros | Corpus Christi (AA) | Activated from disabled list |
| Steven Moya | LF | 24.313 | Detroit Tigers | Toledo (AAA) | Assigned to Toledo (AAA) |
| Nick Nelson | p | 20.225 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| James Norwood | P | 22.205 | Chicago Cubs | South Bend (A) | Activated from disabled list |
| T.J. Olesczuk | OF | 24.163 | Baltimore Orioles | Frederick (A+) | Activated from disabled list |
| Jensen Park | of | 23.219 | Colorado Rockies | Asheville (A) | Assigned to Asheville (A) |
| Ricardo Pinto | P | 22.178 | Philadelphia Phillies | Reading (AA) | Reinstated to active roster |
| Christian Powell | P | 25.014 | Houston Astros | Lancaster (A+) | Assigned to Lancaster (A+) |
| Noe Ramirez | RP | 26.207 | Boston Red Sox | Pawtucket (AAA) | Assigned to Pawtucket (AAA) |
| Victor Rey | 3b | 21.018 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Brian Reyes | C | 21.019 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Francisco Rios | P | 21.072 | Toronto Blue Jays | Dunedin (A+) | Reinstated to active roster |
| Yadiel Rivera | SS | 24.076 | Milwaukee Brewers | Colorado Springs (AAA) | Assigned to Colorado Springs (AAA) |
| Angel Rosa | SS | 23.301 | Los Angeles Angels | Salt Lake (AAA) | Assigned to Salt Lake (AAA) |
| Bubby Rossman | P | 24.018 | Los Angeles Dodgers | Rancho Cucamonga (A+) | Activated from disabled list |
| Yolmer Sanchez | 3B | 24.018 | Chicago White Sox | Charlotte (AAA) | Assigned to Charlotte (AAA) |
| Gonzalo Sanudo | P | 24.189 | Houston Astros | Corpus Christi (AA) | Assigned to Corpus Christi (AA) |
| Zack Short | if | 21.049 | Chicago Cubs | Eugene (A-) | Assigned to Eugene (A-) |
| Blake Smith | p | 23.339 | Los Angeles Angels | Burlington (A) | Assigned to Burlington (A) |
| Kyle Smith | of | 22.276 | Arizona Diamondbacks | Missoula (Rk) | Assigned to Missoula (Rk) |
| Josh Staumont | p | 22.208 | Kansas City Royals | Northwest Arkansas (AA) | Assigned to Northwest Arkansas (AA) |
| Michael Strentz | C | 24.250 | Los Angeles Angels | Inland Empire (A+) | Assigned to Inland Empire (A+) |
| Zach Thompson | P | 22.267 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Dylan Tice | 2b | 23.214 | St. Louis Cardinals | Peoria (A) | Assigned to Peoria (A) |
| Matt Tindall | p | 24.062 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Chase Vallot | C | 19.330 | Kansas City Royals | Lexington (A) | Assigned to Lexington (A) |
| Pedro Vasquez | p | 20.298 | Seattle Mariners | Clinton (A) | Assigned to Clinton (A) |
| Zak Wasserman | 1B | 25.321 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Justin Williams | SS | 20.332 | Tampa Bay Rays | Montgomery (AA) | Assigned to Montgomery (AA) |
| Josh Wirsu | P | 22.316 | St. Louis Cardinals | Peoria (A) | Assigned to Peoria (A) |
| Matt Wotherspoon | P | 24.285 | New York Yankees | Trenton (AA) | Assigned to Trenton (AA) |
| Asher Wojciechowski | P | 27.208 | Miami Marlins | New Orleans (AAA) | Designated for Assignment |
| Clayton Blackburn | P | 23.192 | San Francisco Giants | Sacramento (AAA) | DL-7 Injured |
| Austin Voth | P | 24.021 | Washington Nationals | Syracuse (AAA) | Placed on temporary inactive list |
| Emerson Landoni | 2B | 27.148 | Atlanta Braves | Gwinnett (AAA) | DL-7 Injured |
| Jeremy Bleich | P | 29.029 | | | Released by Phillies |
| Miguel Urena | p | 21.140 | Los Angeles Dodgers | Ogden (Rk) | DL-60 Injured |
| Ricky Oropesa | 1B | 26.214 | San Francisco Giants | Sacramento (AAA) | DL-7 Injured |
| Angel Villalona | IF | 25.338 | San Francisco Giants | Richmond (AA) | DL-7 Injured |
| Brandon Woodruff | P | 23.157 | Milwaukee Brewers | Biloxi (AA) | Placed on temporary inactive list |
| Brett Lee | P | 25.300 | | | Released by Brewers |
| Glynn Davis | CF | 24.223 | Baltimore Orioles | Bowie (AA) | DL-7 Injured |
| Tucker Nathans | IF | 27.253 | Baltimore Orioles | Bowie (AA) | DL-7 Injured |
| Tyler Horan | OF | 25.227 | San Francisco Giants | Richmond (AA) | DL-7 Injured |
| Cody Dent | SS | 24.351 | | | Released by Nationals |
| Cody Reed | P | 20.040 | Arizona Diamondbacks | Visalia (A+) | DL-7 Injured |
| Dominic Blanco | C | 20.250 | | | Released by Mariners |
| Mike Estevez | p | 23.293 | Toronto Blue Jays | Bluefield (Rk) | DL-60 Injured |
| Chad De La Guerra | 2b | 23.235 | Boston Red Sox | Greenville (A) | DL-7 Injured |
| Cody Gunter | 3B | 22.090 | Washington Nationals | Hagerstown (A) | DL-7 Injured |
| Jack Goihl | c | 23.137 | Cleveland Indians | Lake County (A) | DL-7 Injured |
| Jordan Romano | P | 23.087 | Toronto Blue Jays | Lansing (A) | DL-7 Injured |
| Luis Torres | P | 22.043 | | | Released by Nationals |
| Ramire Cleto | P | 23.104 | | | Released by Mariners |
| Reece Karalus | p | 22.033 | Tampa Bay Rays | Bowling Green (A) | DL-7 Injured |
| Taylor Hawkins | C | 22.303 | Tampa Bay Rays | Bowling Green (A) | DL-7 Injured |
| Victor Gonzalez | P | 20.244 | Los Angeles Dodgers | Great Lakes (A) | DL-7 Injured |
| Yoiber Marquina | C | 20.165 | Cleveland Indians | Lake County (A) | DL-7 Injured |
| Brandon Stephens | p | 24.051 | Seattle Mariners | Arizona Mariners (Rk) | DL-60 Injured |
| Grofi Cruz | SS | 20.105 | | | Released by Indians |
| Gunner Pollman | c | 21.164 | Miami Marlins | Gulf Coast Marlins (Rk) | DL-7 Injured |
| Hank Morrison | of | 22.114 | Minnesota Twins | Elizabethton (Rk) | DL-7 Injured |
| Jonas Lantigua | 1B | 21.214 | Seattle Mariners | Arizona Mariners (Rk) | DL-60 Injured |
| Logan Nottebrok | 3b | 24.115 | | | Announced retirement |
| Braxton Garrett | p | 18.346 | Miami Marlins | Gulf Coast Marlins (Rk) | Signed out of draft (Alabama HS) |
| Mike Shawaryn | p | 21.303 | Boston Red Sox | Lowell (A-) | Signed out of draft (Maryland) |
| Ted Hammond | p | 22.212 | Seattle Mariners | Arizona Mariners (Rk) | Signed as undrafted free agent (Seattle) |
| Warwick Saupold | RP | 26.182 | Detroit Tigers | Toledo (AAA) | Activated from DL and assigned to Toledo (AAA) |
