| Keith Castillo | C | 25.045 | Tampa Bay Rays | Bowling Green (A) | Activated from DL |
| Logan Kensing | P | 30.052 | Pittsburgh Pirates | Indianapolis (AAA) | Activated from DL |
| Gaspar Santiago | P | 22.335 | San Francisco Giants | Salem-Keizer (A-) | Activated from DL |
| Ji-Man Choi | 1B-C | 21.097 | Seattle Mariners | Clinton (A) | Activated from DL |
| Duanel Jones | 3B | 19.105 | San Diego Padres | Fort Wayne (A) | Activated from DL |
| Jalen Goree | SS | 19.070 | San Diego Padres | Arizona Padres (Rk) | Activated from DL |
| Kyle Waldrop | RP | 26.301 | Minnesota Twins | Minnesota Twins (MLB) | Recalled from Rochester (AAA) |
| CC Sabathia | SP | 32.034 | New York Yankees | New York Yankees (MLB) | Activated from DL |
| Jesse Chavez | P | 29.003 | Oakland Athletics | Oakland Athletics (MLB) | Traded from Blue Jays to Athletics for cash |
| Jim Miller | RP | 30.118 | Oakland Athletics | Oakland Athletics (MLB) | Recalled from Sacramento (AAA) |
| Tyson Ross | SP-RP | 25.124 | Oakland Athletics | Sacramento (AAA) | Optioned to Sacramento (AAA) |
| Luis Hernandez | ss | 28.059 | Texas Rangers | Texas Rangers (MLB) | Contract purchased from Round Rock (AAA) |
| Michael Young | dh | 35.309 | Texas Rangers | Texas Rangers (MLB) | Placed on Paternity Leave |
| Jose Bautista | rf | 31.309 | Toronto Blue Jays | Toronto Blue Jays (MLB) | Activated from DL |
| Chad Beck | RP | 27.219 | Toronto Blue Jays | Las Vegas (AAA) | Optioned to Las Vegas (AAA) |
| Brian Schneider | c | 35.271 | Philadelphia Phillies | Philadelphia Phillies (MLB) | DL-15 Strained left hamstring |
| Daniel McCutchen | P | 29.332 | Pittsburgh Pirates | Indianapolis (AAA) | Optioned to Indianapolis (AAA) |
| Jordy Mercer | ss | 25.362 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | Activated from Paternity Leave List |
| Barret Browning | RP | 27.239 | St. Louis Cardinals | Memphis (AAA) | Optioned to Memphis (AAA) |
| Brandon Dickson | RP | 27.294 | St. Louis Cardinals | St. Louis Cardinals (MLB) | Recalled from Memphis (AAA) |
| Shaun Marcum | SP | 30.253 | Milwaukee Brewers | Milwaukee Brewers (MLB) | Activated from DL |
| Yamaico Navarro | lf | 24.298 | Pittsburgh Pirates | Indianapolis (AAA) | Optioned to Indianapolis (AAA) |
| Frank Herrmann | RP | 28.086 | Cleveland Indians | Columbus (AAA) | Optioned to Columbus (AAA) |
| Matt Carson | rf | 31.054 | Minnesota Twins | Rochester (AAA) | Optioned to Rochester (AAA) |
| Hisanori Takahashi | RP | 37.124 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | Claimed off waivers from Angels |
| Jeff Clement | 1b | 29.003 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | Contract purchased from Indianapolis (AAA) |
| Matt LaPorta | dh | 27.228 | Cleveland Indians | Cleveland Indians (MLB) | Recalled from Columbus (AAA) |
| Ryan Flaherty | 2b | 26.028 | Baltimore Orioles | Baltimore Orioles (MLB) | Activated from DL |
| Tommy Hunter | SP | 26.052 | Baltimore Orioles | Norfolk (AAA) | Optioned to Norfolk (AAA) |
| Brian Matusz | RP | 25.194 | Baltimore Orioles | Baltimore Orioles (MLB) | Recalled from Norfolk (AAA) |
| J.C. Romero | RP | 36.081 | Baltimore Orioles | Baltimore Orioles (MLB) | Designated for assignment |
| David Ortiz | dh | 36.280 | Boston Red Sox | Boston Red Sox (MLB) | Activated from DL |
| Junichi Tazawa | RP | 26.079 | Boston Red Sox | Pawtucket (AAA) | Optioned to Pawtucket (AAA) |
| Leyson Septimo | RP | 27.048 | Chicago White Sox | Charlotte (AAA) | Assigned to Charlotte (AAA) for rehab |
| Alex Castellanos | RF | 26.020 | Los Angeles Dodgers | Los Angeles Dodgers (MLB) | Recalled from Albuquerque (AAA) |
| Rubby De La Rosa | RP | 23.173 | Los Angeles Dodgers | Albuquerque (AAA) | Optioned to Albuquerque (AAA) |
| Chad Tracy | 1b | 32.094 | Washington Nationals | Washington Nationals (MLB) | Signed to 1-year contract extension thru 2013 |
| Jeremy Hefner | SP-RP | 26.166 | New York Mets | New York Mets (MLB) | Activated from Paternity Leave List |
| Collin McHugh | SP | 25.066 | New York Mets | Buffalo (AAA) | Optioned to Buffalo (AAA) |
| Steve Lerud | c | 27.315 | Philadelphia Phillies | Philadelphia Phillies (MLB) | Contract purchased from Reading (AA) |
| Josh Spence | P | 24.215 | San Diego Padres | San Diego Padres (MLB) | Recalled from Tucson (AAA) |
| Dale Thayer | CL | 31.250 | San Diego Padres | San Diego Padres (MLB) | Placed on Paternity Leave |
| Cesar Jimenez | P | 27.285 | Seattle Mariners | Tacoma (AAA) | Activated from DL |
| David Pauley | P | 29.068 | Seattle Mariners | Tacoma (AAA) | Suspended 50 games for drug of abuse |
| Jon Link | P | 28.154 | Miami Marlins | New Orleans (AAA) | Placed on temporarily inactive list |
| Matt Clark | LF-1B | 25.257 | San Diego Padres | Tucson (AAA) | Activated from DL |
| Sean White | P | 31.121 | Los Angeles Angels | Salt Lake (AAA) | Activated from DL |
| Steven Wright | P | 27.359 | Boston Red Sox | Pawtucket (AAA) | Activated from DL |
| Adam Abraham | 3B | 25.150 | Cleveland Indians | Akron (AA) | Activated from DL |
| Chris Balcom-Miller | P | 23.174 | Boston Red Sox | Portland (AA) | DL-7 Injured |
| Chris Reed | P | 22.096 | Los Angeles Dodgers | Chattanooga (AA) | Activated from DL |
| Troy Tulowitzki | SS | 27.318 | Colorado Rockies | Tulsa (AA) | Assigned to Tulsa (AA) for rehab |
| Kevin Kouzmanoff | 3B | 31.030 | Kansas City Royals | Northwest Arkansas (AA) | DL-7 Injured |
| Josh Zeid | P | 25.153 | Houston Astros | Corpus Christi (AA) | Activated from DL |
| Adam Bailey | RF | 24.171 | Houston Astros | Corpus Christi (AA) | DL-7 Injured |
| Yordano Ventura | P | 21.082 | Kansas City Royals | Northwest Arkansas (AA) | Activated from DL |
| Lay Batista | P | 23.020 | Los Angeles Angels | Inland Empire (A+) | DL-7 Strained right bicep |
| Jack Leathersich | P | 22.041 | New York Mets | St. Lucie (A+) | Placed on temporarily inactive list |
| Rylan Sandoval | 2B | 25.014 | New York Mets | St. Lucie (A+) | Activated from DL |
| John Taylor | P | 23.150 | Seattle Mariners | Clinton (A) | DL-7 Injured |
| DJ Driggers | OF | 20.057 | Detroit Tigers | Gulf Coast Tigers (East) (Rk) | Suspended 50 games for positive test of Drostanolone |
| Lance Berkman | 1b | 36.196 | St. Louis Cardinals | Memphis (AAA) | Assigned to Memphis (AAA) for rehab |
| Anthony Gallas | LF | 24.254 | Cleveland Indians | Columbus (AAA) | Assigned to Columbus (AAA) |
| Jhonatan Solano | c | 27.012 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) for rehab |
| Ben Kline | IF | 23.265 | Tampa Bay Rays | Bowling Green (A) | |
| Mark Haddow | RF | 24.256 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Shannon Wilkerson | LF-CF | 24.035 | Boston Red Sox | Portland (AA) | Assigned to Portland (AA) |
| Torsten Boss | IF | 21.240 | Baltimore Orioles | Aberdeen (A-) | Assigned to Aberdeen (A-) |
| Sean Jamieson | SS | 23.175 | Oakland Athletics | Burlington (A) | Assigned to Burlington (A) |
| Sean Jamieson | SS | 23.175 | Arizona Diamondbacks | South Bend (A) | Assigned to South Bend (A) |
| Tyler Tewell | C | 21.038 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Austin Cowen | IF | 22.343 | Los Angeles Dodgers | Ogden (Rk) | Assigned to Ogden (Rk) |
| Pedro Feliciano | P | 35.364 | New York Yankees | Tampa (A+) | Assigned to Tampa (A+) for rehab |
| Edgar Gonzalez | SS-2B | 29.182 | Houston Astros | Oklahoma City (AAA) | Assigned to Oklahoma City (AAA) |
| AJ Battisto | P | 28.329 | Miami Marlins | Gulf Coast Marlins (Rk) | |
| Drew Carpenter | RP | 27.098 | New York Mets | Buffalo (AAA) | Assigned to Buffalo (AAA) |
| Kevin Pucetas | P | 27.270 | Washington Nationals | Harrisburg (AA) | Assigned to Harrisburg (AA) |
| Santo Manzanillo | P | 23.247 | Milwaukee Brewers | Wisconsin (A) | Assigned to Wisconsin (A) |
| Thad Weber | P | 27.330 | San Diego Padres | Tucson (AAA) | Assigned to Tucson (AAA) |
| Max Peterson | P | 24.058 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Will Hudgins | P | 22.193 | Washington Nationals | Auburn (A-) | Assigned to Auburn (A-) |
| Christopher Hanna | P | 20.170 | Texas Rangers | Hickory (A) | Assigned to Hickory (A) |
| Justin Ennis | P | 24.126 | Pittsburgh Pirates | West Virginia (A) | Assigned to West Virginia (A) |
| Emmanuel De Leon | P | 21.242 | Pittsburgh Pirates | Bradenton (A+) | Assigned to Bradenton (A+) |
| Jeremy Gigliotti | P | 24.221 | San Diego Padres | Fort Wayne (A) | Assigned to Fort Wayne (A) |
| Eswarlin Jimenez | P | 20.271 | Los Angeles Angels | Inland Empire (A+) | Assigned to Inland Empire (A+) |
| Jason Wilson | P | 22.018 | Tampa Bay Rays | Bowling Green (A) | Assigned to Bowling Green (A) |
| David Otterman | P | 21.101 | Milwaukee Brewers | Arizona Brewers (Rk) | Assigned to Arizona Brewers (Rk) |
| Cesar Vargas | | 20.238 | New York Yankees | Charleston (sc) (A) | Assigned to Charleston (Sc) (A) |
| Jose Brazoban | | 19.088 | Kansas City Royals | Kane County (A) | Assigned to Kane County (A) |
| Bill Rhinehart | RF | 27.275 | | | Released by Reds |
| Ross Wilson | LF-2B | 23.288 | Chicago White Sox | Winston-Salem (A+) | DL-7 Injured |
| Stefan Sabol | C | 20.204 | New York Mets | Brooklyn (A-) | DL-7 Injured |
